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صفحه اصلی
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اولین کنگره بین المللی رویکردهای نوین سبک زندگی، پیشگیری و درمان سرطان
Phycocyanin lead to a reduction of human gastric cancer cells proliferation through the decrease of Bcl-2 expression
نویسندگان :
MARYAM LOTFI (انستیتو پاستور ایران -پایگاه امل) , Seyed Reza Tabaripoor (دانشگاه آزاد اسلامی بابل) , Mohsen Asouri (انستیتو پاستور ایران -پایگاه امل) , Sadegh FATTAHI (انستیتو پاستور ایران -پایگاه امل) , Galia Amirbozorgi (انستیتو پاستور ایران -پایگاه امل) , Saeid Kavoosian (انستیتو پاستور ایران -پایگاه امل) , EFFAT Hayati (انستیتو پاستور ایران -پایگاه امل)
کلمات کلیدی :
Phycocyanin،Gastric cancer،Sonic hedgehog،apoptosis،anti-tumor
چکیده :
The third greatest cause of cancer-related death worldwide is gastric cancer (GC). Many traditional GC treatments, including as chemotherapy and surgery, have numerous side effects and limit treatment effectiveness. As a result, it appears that applying new therapeutic drugs with fewer side effects and higher efficacy is important. In a number of cancer cell lines, phycocyanin from Spirulina Platensis has anti-tumor properties. However, the molecular mechanism of phycocyanin action remains unknown. The goal of this study was to determine the anticancer activity of phycocyanin from Spirulina platensis extract on MKN45 gastric cancer cell line, as well as its effect on the expression of Gli1 from the sonic hedgehog (Shh) signaling pathway, and Bcl-2 as a GLI1 target gene. Cytotoxicity and antiproliferation activity were assessed using the MTT test with various doses of phycocyanin and various incubation durations and stem-loop Taqman real-time PCR assay was used to examine GLI1 and Bcl-2 gene expression. Assays revealed that phycocyanin had a significant influence on gastric cancer cells (MK45) by decreasing proliferation and causing apoptosis via reducing the expression of the anti-apoptotic gene Bcl-2. Furthermore, phycocyanin has no effect on Bcl-2 genes in normal cells, which is significant for employing phycocyanin in cancer treatments to prevent unwanted side effects. Finally, the findings suggest that phycocyanin selectively triggers apoptosis in MK45 gastric cancer cell line by modifying the expression of the anti-apoptotic gene to some extent, which could lead to improved gastric cancer medication.
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