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صفحه اصلی
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اولین کنگره بین المللی رویکردهای نوین سبک زندگی، پیشگیری و درمان سرطان
Phycocyanin has good potential to inhibit cell proliferation by induction of G0/G1 phase in human squamous carcinoma cell line
نویسندگان :
Effat Hayati (انستیتو پاستور ایران) , Reza Tabaripoor (دانشگاه آزاد اسلامی واحد بابل) , Mohsen Asouri (انستیتو پاستور ایران) , Sadegh Fattahi (انستیتو پاستور ایران) , Maryam Lotfi (انستیتو پاستور ایران) , Sara Ghobadi Rad (انستیتو پاستور ایران)
کلمات کلیدی :
Cancer therapy،Phycocyanin،Lung cancer،Cell cycle arrest،G0/G1 phase
چکیده :
Background: Squamous cell lung cancer (SCC) is a type of non-small cell lung cancer (NSCLC), and it is the second most frequent type of lung cancer. It accounts for around 30% of all non-small-cell lung cancer cases. Despite the enormous advancements in lung cancer therapy, significant gaps in the development of new medicines persist. Phycocyanin, a blue pigment with antioxidative and fluorescent properties, has been extensively studied in the therapy of NSCLC cell lines such as A549, H460, H1299, and LTEP-a2. The effects of varying phycocyanin concentrations on viability, death pattern, and expression of the SUFU and bcl-2 genes in normal human dermal fibroblast (HDF) and human squamous carcinoma cell lines (SK-MES-1) were investigated in this work. Methods: The two cell lines In the appropriate media, SK-MES-1 and the HDF normal cell line were cultivated, and various amounts of phycocyanin (172, 375, 750, and 1500 g/ml) were added. After 24 and 48 hours, the MTT assay was used to evaluate metabolic activity. SUFU and BCL-2 gene expression were evaluated by a TaqMan real-time PCR assay, and cell cycle analysis is often carried out using flow cytometry to identify cells in different phases of the cell cycle (G0/G1 vs. S vs. G2/M). Results: After 24 and 48 hours, phycocyanin significantly inhibits the proliferation of SK-MES-1 cells (P≤0.001). Under treatment with various doses of phycocyanin, SK-MES-1 cells showed reduced SUFU and BCL-2 expression levels. Additionally, phycocyanin treatment caused the G0/G1 phase of the cell cycle to be arrested in SK-MES-1 cell lines. Conclusion: Our findings imply that phycocyanin can influence human squamous carcinoma cell lines and alter their proliferation rate by altering the expression of the SUFU and BCL-2 genes and inducing the G0/G1 phase following treatment. As a result, phycocyanin has the potential to be used as a therapeutic agent for lung cancer.
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