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صفحه اصلی
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اولین کنگره بین المللی رویکردهای نوین سبک زندگی، پیشگیری و درمان سرطان
Multiplex Real-Time PCR Diagnostic Panel for Human Papillomavirus Type 16 Viral Load and Genome Physical Status Determination
نویسندگان :
فرزین صادقی (دانشگاه علوم پزشکی بابل)
کلمات کلیدی :
Human papillomavirus 16،Low Grade Squamous Intraepithelial Lesion،High Grade Squamous Intraepithelial Lesion،Squamous Cell Carcinoma،Viral Load
چکیده :
Background: Human Papillomavirus type 16 (HPV 16) E2 and E6 genes viral load and physical condition of HPV-16 genome have been proposed as indicator biomarkers for development of cervical lesions into malignancies. This study examined the viral load and physical status of the HPV-16 genome in non-cancerous, precancerous and cancerous cervical lesions. Methods: Multiplex real-time PCR was developed to determine HPV-16 E2 and E6 viral load in 132 cervical specimens. E2/E6 viral load ratio was used to determine the physical status of HPV-16 genome. Results: E2 gene viral load was a significant (P <0.001) predictor biomarker in differentiating non-cancerous from precancerous and cancerous samples. E6 gene viral load was significantly different between groups (P <0.001). The specificity and sensitivity of E2 and E6 in distinguishing SCC samples were 100% and 95%. Conclusion: HPV-16 viral load measured through E2 and E6 genes is a reliable indicator of lesion type. Legal entity responsible for the study: The authors Funding: This work was funded and supported by Babol University of Medical Sciences Disclosure: Al authors have declared no conflict of interests
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