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صفحه اصلی
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اولین کنگره بین المللی رویکردهای نوین سبک زندگی، پیشگیری و درمان سرطان
Chrysin potential on the amelioration of Cyclophosphamide toxic effects on mouse oocytes and embryos
نویسندگان :
Sima Etebari (Sabzevar University of Medical Science, Sabzevar, Iran) , Ali Asghar Ahmadi (انستیتو پاستور ایران)
کلمات کلیدی :
Chrysin،Cyclophosphamide،Oocyte collection،Oocyte quality
چکیده :
Objective: The study investigates the protective effects of chrysin (CR) on the quality of oocytes and embryos of in vitro fertilization in rats treated with cyclophosphamide (CPH). Materials and Methods: This study, female NMRI mice were divided into five groups: I. control group, II. sham group, III. CPH group (receiving 120 mg/kg.wk of CPH intraperitoneally (IP), IV and V: CR groups receiving 5 and 10 mg/kg. day CR for four weeks. For oocyte induction, ten units of pregnant mare serum gonadotropin was injected intraperitoneally after the last injection. All mice were then sacrificed by aspiration of their oocytes for further experiments. The growth of embryos was investigated using mature oocytes in vitro. Results: CR significantly increased the number of 2 cells and 4 cells after 24 and 48 hours compared to the CPH group. Groups treated with CR showed a significant increase in the expression level of BMP-15 and GDF-9 gene in a dose-dependent manner compared to the CPH group. Conclusion: In mice, CR reduced oxidative damage and oocyte cytokine levels in ovarian tissue after CPH-induced degeneration.
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