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صفحه اصلی
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اولین کنگره بین المللی رویکردهای نوین سبک زندگی، پیشگیری و درمان سرطان
بررسی خاصیت آنتی اکسیدانی و ضد سرطانی عصاره برگ گیاه آقطی (L. Sambucus ebulus) بر روی سلول سرطان ریه رده A549
نویسندگان :
امین توکلی یرکی (دانشگاه آزاد اسلامی تنکابن) , سیمین آرین (دانشگاه آزاد اسلامی تنکابن) , مهدیه هوشنی (دانشگاه آزاد اسلامی واحد تنکابن) , آذر زمانی چیرپردی (دانشگاه آزاد اسلامی واحد تنکابن)
کلمات کلیدی :
آقطی،ترکیبات آنتی اکسیدان،سنجش MTT،رده سلولی A549
چکیده :
Introduction: Cancer considers the one of the most reasons of mortality in worldwide. Components derived from natural plants that induce apoptosis are used for cancer treatment. Thus, the investigation of different herbal components for new anti-cancer drug is one of the main research activities throughout the world. Therefore, the purpose of this research is to investigate the antioxidant compounds of the extract of the Sambucus ebulus leaf and its inhibitory effect on A549 lung cancer cells. Materials and Methods: Leaf of Sambucus ebulus was harvested from the Abbas Abad city and then dried in shade and extracted. In the following, A549 cells line was cultivated and proliferated. Then, the cells exposed to different concentrations of the leaf extract (31.25 to 4000 μg/ml) were incubed for 24, 48, and 72 hours. After the incubation period, the colorimetric MTT method was used to determine cytotoxicity. Also, total phenol, flavonoid, and anthocyanin contents were determined. Results: The results indicated that the leaf extract is rich in phenol, flavonoid, and total anthocyanin compounds, so that the amount of these compounds in the leaf extract was obtained 22.41±0.883, 1.29±0.016, and 0.193±0.060 mg-1g DW, respectively.Also, compared to control, the results reported that the different concentrations of leaf and root extracts had significantly reduced cell viability (p˂0.05). The effect of extracts on cell growth inhibition was dependent on concentration and time, and the highest of cell growth inhibition were o obtained at the concentration level of 4000 μg /ml leaf extract 97.47% in 72 hours, respectively. Conclusion: The results of this study suggest that the leaf extract of Sambucus ebulus plant can be an accessible source of antioxidant compounds and with high anti-cancer properties it can be used as a medicinal plant against lung cancer subject to extensive research.
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