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صفحه اصلی
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اولین کنگره بین المللی رویکردهای نوین سبک زندگی، پیشگیری و درمان سرطان
Examining the tumorigenic pathology results of Lactobacillus acidophilus bacterium supernatant effect on MCF7 cell line breast cancer mouse model
نویسندگان :
Ramezan Behzadi (دانشگاه آزاد علوم تحقیقات تهران)
کلمات کلیدی :
Lactobacillus acidophilus،supernatant،mouse xenograft model،MCF7 cell line
چکیده :
Examining the tumorigenic pathology results of Lactobacillus acidophilus bacterium supernatant effect on MCF7 cell line breast cancer mouse model Ramzan Behzadi1, 2, Ismail Fatahi3, Fatemeh Zabali3, Khaled Saidi4, Rana Jahanban Esfahalan4, Alireza Salehi5, Sajjad Ahmadpour6, Karim Eyvazi Atashbeyk6, Ahmed Harmati7 1- North Research School of Pasteur Institute of Iran, Amol, Mazandaran, Iran 2- Department of Biology, Faculty of Science, Science and Research Unit, Islamic Azad University, Tehran, Iran 3- Department of Biology, Amol Unit, Islamic Azad University, Amol, Iran 4- Tabriz University of Medical Sciences, Tabriz, Iran 5- Department of Pathobiology, Babol Branch, Islamic Azad University, Babol, Iran 6- Digestive and Liver Diseases Research Center, Qom University of Medical Sciences, Qom, Iran 7- Department of Orthopedics, Urmia University of Medical Sciences, Urmia, Iran Abstract Introduction Breast cancer is the most common cancer diagnosed in women worldwide with 2.26 million new cases in 2020. In the eastern Mediterranean region, including Iran, breast cancer has the highest incidence and mortality rates among women compared to other cancers. Moreover, cancer is the second cause of human death in the world, next to the cardiovascular diseases. Based on previous research, using probiotic products has beneficial effects on human health through maintaining the intestinal microbial balance. Methods: In this research, the effect of subcutaneous administration of lactobacillus acidophilus bacterium supernatant on MCF7 breast cancer mouse xenograft model was investigated. Results: Our histopathological examination showed that lactobacillus acidophilus supernatant prevented and reduced tumor growth. It was observed that the growth was depended on the applied dose of the supernatant, where tumor growth inhibition was decreased significantly by increasing the concentration of supernatant. Furthermore, With increasing the dose or increasing the concentration of lactobacillus acidophilus supernatant, vacuolar degeneration, hyperemia, infiltration of amyloid cells and replacement of connective tissue were observed. The degree of vacuolar degeneration was observed in the 5% group as mild and in the 10% and 20% group as moderate. Moderate hyperemia was observed only in the 20% group. It seems that increasing the dose of supernatant caused changes in osmotic pressure in cancer cells and vacuolar accumulations. Conclusion: First, it can be concluded according to the histopathological results, the best effect was observed in 20%. Second, it is proposed that probiotics have less side effects and toxicity than chemical drugs due to the presence of natural and effective compounds and can be introduced as a new strategy for cancer treatment. Key words: Lactobacillus acidophilus, supernatant, mouse xenograft model, MCF7 cell line
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