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صفحه اصلی
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اولین کنگره بین المللی رویکردهای نوین سبک زندگی، پیشگیری و درمان سرطان
The effect of thymoquinone on oogenesis and the oocyte in vitro fertilization success in NMRI mice treated with cyclophosphamide
نویسندگان :
Sima Etebari (Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran) , Ali Asghar Ahmadi (انستیتو پاستور ایران)
کلمات کلیدی :
Thymoquinone،Oocyte quality،Cyclophosphamide،Fertilized embryos
چکیده :
Objective: The study objective was to determine the protective effects of thymoquinone (TQ) on oocyte quality in oogenesis and the quality of in vitro fertilization embryos in NMRI mice treated with cyclophosphamide. Materials and Methods: Female NMRI mice were divided into five groups. All mice were sacrificed after that to aspirate their oocytes for further testing. The development of embryos up to the blastocyst stage was analyzed using mature oocytes in vitro fertilization (IVF). Finally, the oocytes were removed for biochemical assessments. SPSS and Mann-Whitney tests were used to analyze the data. Results: TQ groups showed a decrease in oocyte degeneration compared to CPH groups, dose-dependently. TQ is safe for oocytes as evidenced by the increased numbers during the GV stage. A dose-dependent lower rate of metaphase I maturation was also observed in the TQ treatment groups compared to CPH, and fewer oocytes were maturing in the phase II stage. In this study, TQ significantly increased the number of two-cell and four-cell embryos after 24 and 48 hours of fertilization in comparison to the CPH group. Additionally, dose-dependently, CAT levels and MDA and NO levels were significantly higher and lower in the TQ groups as compared to the CPH groups. TQ-treated groups showed significant increases in levels of gene expression for BMP15 and GFDF-9, dose-dependently, compared to CPH groups. Conclusion: In the current study, TQ attenuated oocyte cytokine levels and oxidative damage in ovarian tissues after CPH-induced oocyte degeneration in mice.
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ثمین همایش، سامانه مدیریت کنفرانس ها و جشنواره ها - نگارش 35.4.1
