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صفحه اصلی
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اولین کنگره بین المللی رویکردهای نوین سبک زندگی، پیشگیری و درمان سرطان
Investigating expression level of BCL-2 gene under treatment with a novel Liposomal Indole Compound on the AML Cell Line
نویسندگان :
Elham sadat Hosseini (Rafsanjan University of Medical Sciencess) , Mohamad Hasan Sheikha (Shahid Sadoughi University of Medical Sciences) , Bibi Fatemeh Haghiralsadat (Shahid Sadoughi University of Medical Sciences) , Fateme Montazeri (Sadoughi University of Medical Sciences) , Seyed Mehdi Hoseini (Sadoughi University of Medical Sciences) , Gholamhoseini Hasanshahi (Rafsanjan University of Medical Sciencess)
کلمات کلیدی :
AML،BCL-2،Indole،Liposome،Apoptosis،Cancer Therapy،Nanobiotechnology
چکیده :
Background: Acute myeloid leukemia (AML) is a malignant disease that prevents differentiation of the myeloid lineage. There are different types of treatment strategies including chemotherapy, surgery and radiation for curing AML but the typical method is chemotherapy. Unfortunately, older and weak patients might not be able to endure sever chemotherapy; Hence, they need more effective treatment protocols. Also, the quality and response rate of alternative treatment methods for these patients is low. The big parts of anticancer drugs belong to Heterocyclic compounds. Indole derivatives have bicyclic structure including a six-membered ring which is linked to a five-membered nitrogen-containing pyrrole ring. Research conducted demonstrated that most of indole derivatives have broad anticancer effects on several cancer cell lines. In this study, we introduced a novel indole derivative (2-AITFEI-3-C) with tendency to facilitate apoptosis in KG-1 line. Material and methods: In this study the KG-1 cell line as an AML-originated was cultured into RPMI 1640 media which was supplemented with 10% fetal bovine serum. Once the cells reached the appropriate confluence, they were treated by free and liposomal form of novel indole core-based derivative (C18H10N2F6O) with various concentrations of 20,40,100,200, and 400 µg/mL. The cellular metabolic activity was examined by MTT assay. Finally, gene expression of BAX gene was investigated by q-PCR in order to assessment of apoptotic effect of this new compound. Results: Results of the MTT assay indicated that the lethal effective dose of free indole was 245.1 / g / ml in 24 hours and 164.8 / g / ml in 48 hours. The lethal effective dose of the liposomal system containing indole drug was 47.2 µ g / ml in 24 hours and 40.6 / g / ml in 48 hours. The results indicate that the toxicity of indole is increased due to encapsulation. Moreover, BCL-2 gene expression decreased after treatment with free and liposomal form after 24 and 48 hours but statistical analysis showed that BCL-2 gene expression was just significant under the effect of liposomal form after 48 hours (p<0.05). Conclusion: It can be concluded that this novel indole-core based derivative especially the liposomal form of it may have cytotoxic and antiproliferative impacts by triggering the apoptosis of AML cells and we can consider it as a useful agent to limit the process of tumor progression in the treatment of human malignancies.
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