0% Complete
صفحه اصلی
/
اولین کنگره بین المللی رویکردهای نوین سبک زندگی، پیشگیری و درمان سرطان
Gene expression and cytotoxicity properties of Golestan’s native truffle (Tuber aestivum) on MCF-7 cell lines
نویسندگان :
Mehrab Nasirikenari (Pasteur Institute of Iran, Amol) , Marziyeh Ahmadi Mousaabad (Islamic Azad University of Qaemshahr) , Abbas Ali Dehpour Juybari (Islamic Azad University of Qaemshahr)
کلمات کلیدی :
Breast Cancer،Truffle (Tuber aestivum)،Gene Expression،Cytotoxicity
چکیده :
Background: Truffles are the fruiting bodies of ascomycetes that grow naturally in many countries all over the world. They are known as ancient organic sources with nutritional and therapeutic properties. They are rich in biochemical compounds such as terpenoids, polysaccharides, phenolics, and phytosterols which have anticancer, antioxidant, hepatoprotective, antibacterial, immunomodulatory, and anti-inflammatory. activities (Patel S, 2012). Therefore, the aim of this study was to test and determine anti-cancer properties of black truffle (Tuber aestivum) collected from Golestan province, Iran, yet unexplored in terms of its cytotoxic effects on breast cancer cells. Methods: MTT assay was utilized to evaluate cytotoxicity. To deduce the molecular mechanism of Truffle, apoptosis-related genes including Bcl-2 and Bax were evaluated by real-time RT-PCR. The expression changes of the pro-apoptotic Bax gene and the anti-apoptotic Bcl-2 gene and the Bax/Bcl2 ratio were studied through the 2ΔΔCT method. Result: T. aestivum extracts prevented the development of cancer cell lines in a dosage and time dependent manner. Furthermore, when compared to control cell lines (HDF), the truffle extract was the most effective inhibitor against breast cancer cells line (MCF7). To give an illustration, following truffle treatment, a significant reduction in cell viability was seen in cancer cells, although cell vitality in normal control cells was lower. Analysis showed the lowest effective IC50 values of 4356.0 and 6936.8, for cancer and normal cell lines respectively. The truffle action on apoptotic gene expression in normal and cancer cells revealed that this extract significantly increased the expression of pro-apoptotic Bax gene and decreased the expression of inhibitor of apoptosis, Bcl2 gene, in cancer cells compared to normal cells. (p<0.05).
لیست مقالات
ثمین همایش، سامانه مدیریت کنفرانس ها و جشنواره ها - نگارش 34.4.5
